Faculty of Agriculture, The University of Tokyo, Japan, B. S., 2002
2002-2007
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. Ph. D., 2007
Research and Professional Experience
2007-2010
Postdoctoral Fellow, RIKEN, Japan.
2010-2012
Special Postdoctoral Researcher, RIKEN, Japan.
2012-present
Staff Scientist, RIKEN, Japan.
Awards and Professional Activities
2010-2012
Fellowship for Special Postdoctoral Researcher at RIKEN
2011-2013
Grant-in-Aid for Scientific Research on Innovative Areas
2011-2014
Grant-in-Aid for Young Investigators
2018-2021
Grant-in-Aid for Scientific Research
2003-
Member, The Molecular Biology Society of Japan
2004-
Member, Yeast genetics Society of Japan
2005-
Member, Japan Society for Bioscience, Biotechnology, and Agrochemistry
2016-
Member, The Japanese Society for Epigenetics
Publication List
Balan S*. Iwayama Y. Ohnishi T. Fukuda M. Shirai A. Yamada A. Weirich S. Schuhmacher MK. Dileep KV. Endo T. Hisano Y. Kotoshiba K. Toyota T. Otowa T. Kuwabara H. Tochigi M. Watanabe A. Ohba H. Maekawa M. Toyoshima M. Sasaki T. Nakamura K. Tsujii M. Matsuzaki H. Zhang KYJ. Jeltsch A. Shinkai Y*. Yoshikawa T*.
A loss-of-function variant in SUV39H2 identified in autism-spectrum disorder causes altered H3K9 trimethylation and dysregulation of protocadherin beta-cluster genes in the developing brain.
Mol Psychiatry . 2021 Dec;26(12):7550-7559. [PubMed]
Oya E, Nakagawa R, Yoshimura Y, Tanaka M, Nishibuchi G, Machida S, Shirai A, Ekwall K, Kurumizaka H, Tagami H, Nakayama JI.
H3K14 ubiquitylation promotes H3K9 methylation for heterochromatin assembly.
EMBO Reports. 2019 Oct 4;20(10):e48111.[PubMed]
Matsuyama A, Shirai A, Yoshida M.
Preparation of cell lysates of fission yeast for immunoprecipitation.
Methods Mol Biol. 2018;1721:125-133.[PubMed]
Shirai A. Kawaguchi T. Shimojo H. Muramatsu D. Ishida-Yonetani M. Nishimura Y. Kimura H. Nakayama J. and Shinkai Y.
Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly.
eLife, 2017;6. pii: e25317.[PubMed]
Takahashi H, Suzuki T, Shirai A, Matsuyama A, Domae N, Yoshida M.
Mitochondrial localization of fission yeast manganese superoxide dismutase is required for its lysine acetylation and for cellular stress resistance and respiratory growth.
Biochemical and Biophysical Research Communication. 2011; 406(1): 42-6.[PubMed]
Nishimura S, Arita Y, Honda M, Iwamoto K, Matsuyama A, Shirai A, Kawasaki H, Kakeya H, Kobayashi T, Matsunaga S, Yoshida M.
Marine antifungal theonellamides target 3beta-hydroxysterol to activate Rho1 signaling.
Nature Chemical Biology. 2010; 6(7): 519-26.[PubMed]
Shirai A, Sadaie M, Shinmyozu K, Nakayama J.
Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth.
Journal of Biological Chemistry. 2010; 285(29): 22448-60.[PubMed]
Matsuyama A, Shirai A, Yoshida M.
A novel series of vectors for chromosomal integration in fission yeast.
Biochemical and Biophysical Research Communication. 2008; 374(2): 315-9.[PubMed]
Matsuyama A, Shirai A, Yoshida M.
A series of promoters for constitutive expression of heterologous genes in fission yeast.
Yeast. 2008; 25(5): 371-6.[PubMed]
Shirai A, Matsuyama M, Yashiroda Y, Hashimoto A, Kawamura Y, Arai R, Komatsu Y, Horinouchi S, Yoshida M.
Global analysis of gel mobility of proteins and its use in target identification.
Journal of Biological Chemistry. 2008; 283(16): 10745-52.[PubMed]
Shirai A, Matsuyama A, Yashiroda Y, Arai R, Yoshida M.
Rapid and reliable preparation of total cell lysates from fission yeast. Protocol Exchange DOI: 10.1038/nprot.2006.191.
Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M.
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Nature Biotechnology. 2006; 24(7): 841-7[PubMed]
Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M.
pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast.
Yeast. 2004; 21(15): 1289-305.[PubMed]
