Publications

Publications 2001-

2018

  1. Tsusaka T, Kikuchi M, Shimazu T, Suzuki T, Sohtome Y, Akakabe M, Sodeoka M, Dohmae N, Takashi Umehara T, Shinkai Y*. Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8. Epigenetics and chromatin.[PubMed]
  2. Hirano T*, Koyanagi A, Kotoshiba K, Shinkai Y, Kasai M, Ando T, Kaitani A, Okumura K, Kitaura J. The Fab fragment of anti-IgE C2 domain prevents allergic reactions through interacting with IgE-FcRI complex on rat mast cells. Sci Rep. 2018 Sep 24;8(1):14237. doi: 10.1038/s41598-018-32200-z.[PubMed]
  3. Verbaro DJ, Sakurai N, Kim B, Shinkai Y, Egawa T*. The histone methyltransferase G9a is required for silencing of helper T lineage-associated genes in proliferating CD8 T cells. J Immunol. 2018 May 2. pii: ji1701700. doi: 10.4049/jimmunol.1701700. [PubMed]
  4. Sohtome Y, Shimazu T, Barjau J, Fujishiro S, Akakabe M, Terayama N, Dodo K, Ito A, Yoshida M, Shinkai Y, Sodeoka S.* Unveiling Epidithiodiketopiperazine as a Non-Histone Arginine Methyltransferase inhibitor by Chemical Protein Methylome Analyses. Chem Commun (Camb). 2018 Jul 31. doi: 10.1039/c8cc03907k.[PubMed]
  5. Fukuda K, Okuda A, Yusa K, Shinkai Y*. A CRISPR knockout screen Identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 2018 May 4. pii: gr.227280.117. [PubMed]
  6. Kato M, Takemoto K, Shinkai Y*. A somatic role for the histone methyltransferase Setdb1 in endogenous retrovirus silencing. Nat Commun. 2018 Apr 27;9(1):1683. [PubMed]
  7. Kuroki S, Nakai Y, Maeda R, Okashita N, Akiyoshi M, Yamaguchi Y, Kitano S, Miyachi H, Nakato R, Ichiyanagi K, Shirahige K, Kimura H, Shinkai Y, Tachibana M. Combined Loss of JMJD1A and JMJD1B Reveals Critical Roles for H3K9 Demethylation in the Maintenance of Embryonic Stem Cells and Early Embryogenesis. Stem Cell Reports. 2018 Apr 10;10(4):1340-1354. [PubMed]
  8. Shimazu T, Furuse T, Balan S, Yamada I, Okuno S, Iwanari H, Suzuki T, Hamakubo T, Dohmae N, Yoshikawa T, Wakana S, Shinkai Y*. Role of METTL20 in regulating -oxidation and heat production in mice under fasting or ketogenic conditions.Sci Rep. 2018 Jan 19;8(1):1179 [PubMed]
  9. Yamada A, Shimura C, Shinkai Y*. Biochemical validation of EHMT1 missense mutations in Kleefstra syndrome. J Hum Genet. 2018 Feb 19. [PubMed]
  10. 2017

    1. Ferry L#, Fournier A#, Tsusaka T#, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y*, Defossez PA*. Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation. Mol Cell. 2017 Aug 17;67(4):550-565.e5. #These authors contributed equally [PubMed]
    2. Shirai A. Kawaguchi T. Shimojo H. Muramatsu D. Ishida-Yonetani M. Nishimura Y. Kimura H. Nakayama J*. and Shinkai Y*. Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. eLife, 2017 Aug 1;6. pii: e25317. [PubMed]
    3. Nakatsuka T.Tateishi K*. Kudo Y. Yamamoto K.Nakagawa H,Fujiwara H,Takahashi R. Miyabayashi K. Asaoka Y. Tanaka Y. Ijichi H. Hirata Y. Otsuka M. Kato M. Sakai J. Tachibana M. Aburatani H. Shinkai Y. Koike K. Impact of histone demethylase KDM3A-dependent AP-1 transactivity on hepatotumorigenesis induced by PI3K activation. Oncogene. 2017 Jul 10. [PubMed]
    4. Kamiunten T, Ideno H, Shimada A, Arai Y, Terashima T, Tomooka Y, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A*. Essential roles of G9a in cell proliferation and differentiation during tooth development. Exp Cell Res. 2017 May 17. pii: S0014-4827(17)30294-X. [PubMed]
    5. 2016

      1. Muramatsu D. Kimura H. Kotoshiba K. Tachibana M. and Shinkai, Y*. Pericentric H3K9me3 formation by HP1 interaction-defective histone methyltransferase Suv39h1. Cell Struct Funct. 2016 Dec 3;41(2):145-152. [PubMed]
      2. Koide S, Oshima M, Takubo K, Yamazaki S, Nitta E, Saraya A, Aoyama K, Kato Y, Miyagi S, Nakajima-Takagi Y, Chiba T, Matsui H, Arai F, Suzuki Y, Kimura H, Nakauchi H, Suda T, Shinkai Y, Iwama A*. Setdb1 maintains hematopoietic stem and progenitor cells by restricting the ectopic activation of nonhematopoietic genes. Blood. 2016 Aug 4;128(5):638-649. [PubMed]
      3. Takikita S, Muro R, Takai T, Otsubo T, Kawamura YI, Dohi T, Oda H, Kitajima M, Oshima K, Hattori M, Endo TA, Toyoda T, Weis J, Shinkai Y, Suzuki H*. A Histone Methyltransferase ESET Is Critical for T Cell Development. J. Immnol. 2016, Sep 15;197(6):2269-2279. [PubMed]
      4. Fukuda M, Inoue M, Muramatsu D, Miyachi H, and Shinkai Y*. Knockout mouse production assisted by Blm knockdown. J. Reproduction and Development. 2016, 62:121-125. [PubMed]
      5. Sharif J, Endo TA, Nakayama M, Karimi MM, Shimada M, Katsuyama K, Goyal P, Brind'Amour J, Sun MA, Sun Z, Ishikura T, Mizutani-Koseki Y, Ohara O, Shinkai Y, Nakanishi M, Xie H, Lorincz MC, Koseki H*. Activation of Endogenous Retroviruses in Dnmt1(-/-) ESCs Involves Disruption of SETDB1-Mediated Repression by NP95 Binding to Hemimethylated DNA. Cell Stem Cell. 2016 Jul 7;19(1):81-94. [PubMed]

      2015

      1. Wada S, Ideno H, Shimada A, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A*. H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro. Histochem Cell Biol. 2015 Jul;144(1):13-20. [PubMed]
      2. Collins, P.L. Kyle, K.E. Egawa, T. Shinkai, Y and Oltz, E.M*. The histone methyltransferase SETDB1 represses endogenous and exogenous retroviruses in B lymphocytes. PNAS. 2015 Jul 7;112(27):8367-72. [PubMed]

      2014

      1. Inoue, M. Kuroda, T. Honda, A. Komabayashi-Suzuki, M. Komai, T. Shinkai, Y. and Mizutani, K*. Prdm8 regulates multipolar neuronal precursors in the intermediate zone during neocortical development. PLoS One. 2014 Jan 29;9(1):e86356. doi: 10.1371/journal.pone.0086356. eCollection 2014.[PubMed]
      2. Ueda, J. Lee, K..L. Ho, J. Kitajima, S. Yang, H. Sun, W. Fukuhara, N. Zaiden, N. Chan, S.L. Tachibana, M. Shinkai, Y. Kato, H. and Poellinger, L*. The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. Mol Cell Biol. 2014 Oct 1;34(19):3702-20. doi: 10.1128/MCB.00099-14. Epub 2014 Jul 28.[PubMed]
      3. Shimazu, T. Barjau, J. Sohtome, Y. Sodeoka, M. and Shinkai, Y*. Selenium-based S-adenosylmethionine analog reveals the mammalian seven-beta-strand methyltransferase METTL10 to be an EF1A1 lysine methyltransferase. PLOS ONE 2014 Aug 21;9(8):e105394. doi: 10.1371/journal.pone.0105394.[PubMed]
      4. Balan, S. Iwayama, Y. Maekawa, M. Toyota, T. Ohnishi, T. Toyoshima, M. Shimamoto, C. Esaki, K. Yamada, K. Iwata, Y. Suzuki, K. Ide, M. Ota. M. Fukuchi, S. Tsujii, M. Mori, N. Shinkai, Y. & Yoshikawa, T*. Exon resequencing of H3K9 methyltransferase complex genes, EHMT1, EHMT2 and WIZ, in Japanese autism subjects. Molecular Autism 2014 Oct 6;5(1):49. doi: 10.1186/2040-2392-5-49. eCollection.[PubMed]
      5. Liu S, Brind'Amour J, Karimi MM, Shirane K, Bogutz A, Lefebvre L, Sasaki H, Shinkai Y, Lorincz MC*. Setdb1 is required for germline development and silencing of H3K9me3-marked endogenous retroviruses in primordial germ cells. Genes Dev. 2014 Sep 15;28(18):2041-55. doi: 10.1101/gad.244848.114. [PubMed]

      2013

      1. Deguchi, K. Nagamatsu, G. Miyachi, H. Kato, Y. Morita, S. Kimura, H. Kitano, S. Hatada, I. Saga, Y. Tachibana. M. and Shinkai.Y*. Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male germ cells. Biol. Reprod., 2013, 88:1-8.[PubMed]
      2. Yang, C-M. and Shinkai, Y*. Prdm12 is induced by retinoic acid and exhibits antiproliferative activity of P19 cells. Cell Structure and Function, 2013, 38:195?204. [PubMed]
      3. Kuroki, S. Matoba, S. Akiyoshi, M. Matsumura, Y. Miyachi, H. Mise, N. Abe, K. Kanai, Y. Ogura, A. Wilhelm, D. Koopman, P. Nozaki, M. Shinkai, Y*. and Tachibana, M*. Epigenetic regulation of mouse sex determination by the histone demethylase Jmjd1a. Science, 2013, 341:1106-1109. [PubMed]
      4. Inagawa, M. Nakajima, K. Makino, T. Ogawa, S. Kojima, M. Ito, S. Ikenishi, A. Hayashi, T. Schwartz, R.J. Nakamura, K. Obayashi, T. Tachibana, M. Shinkai, Y. Maeda, K. Miyagawa-Tomita, S. and Takeuchi, T*. Histone H3 Lysine 9 methyltransferases, G9a and GLP are essential for cardiac morphogenesis. Mechanisms of Development, 2013, doi:pii: S0925-4773(13)00057-9. [PubMed]
      5. Kuroki, S. Akiyoshi, M. Tokura, M. Miyachi, H. Nakai, Y. Kimura, H. Shinkai, Y. and Tachibana, M*. JMJD1C, a JmjC domain-containing protein, is required for long-term maintenance of male germ cells in mice. Biol. Reprod., 2013, 89: 93, 1-9. [PubMed]
      6. Balemans MC, Kasri NN, Kopanitsa MV, Afinowi NO, Ramakers G, Peters TA, Beynon AJ, Janssen SM, van Summeren RC, Eeftens JM, Eikelenboom N, Benevento M, Tachibana M, Shinkai Y, Kleefstra T, van Bokhoven H*, Van der Zee CE*. Hippocampal dysfunction in the Euchromatin histone methyltransferase 1 heterozygous knockout mouse model for Kleefstra syndrome. Hum Mol Genet. 2013, 22:852-866. doi: 10.1093/hmg/dds490. [PubMed]

      2012

      1. Takahashi, A. Imai, Y. Yamakoshi, K. Kuninaka, S. Ohtani, N. Yoshimoto, S. Hori, S. Tachibana, M. Anderton, E. Takeuchi, T. Shinkai, Y. Peters, G. Saya, H. and Hara, E*. DNA damage signalling triggers degradation of histone methyltransferases through APC/CCdh1 in senescent cells. Mol Cell. 2012, 45:123-131. [PubMed]
      2. Nakamura, T*. Liu, Y-J. Nakashima, H. Umehara, H. Inoue, K. Matoba, S. Tachibana, M. Ogura, A. Shinkai, Y. and Nakano, T*. PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos. Nature 2012, 486:415-419. [PubMed]
      3. Yamamizu, K. Fujihara, M. Tachibana, M. Katayama, S. Takahashi, A. Hara, E. Imai H. Shinkai, Y. and Yamashita, J.K*. Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a. Cell Stem Cell. 2012, 10:759-770. [PubMed]
      4. Tan, S. Nishi, M. Ohtsuka, T. Matsui, T. Takemoto, K. Kamio-Miura, A. Aburatani, H. Shinkai, S. and Kageyama, R*. Essential roles of the histone methyltransferase ESET in the epigenetic control of neural progenitor cells during development. Development, 2012, 139:3806-3816. [PubMed]
      5. Takahashi M, Takemoto Y, Shimazu T, Kawasaki H, Tachibana M, Shinkai Y, Takagi M, Shin-ya K, Igarashi Y, Ito A, Yoshida M. Inhibition of histone H3K9 methyltransferases by gliotoxin and related epipolythiodioxopiperazines. J Antibiot (Tokyo). 2012, 65:263-265. [PubMed]

      2011

      1. Shinkai, Y. and Tachibana, M.: H3K9 Methyltranferase G9a and the related-molecule GLP. Genes Dev. 2011, 25:781-788. (review) [PubMed]
      2. Leung, D. Dong, K. Maksakova, I. Goyal, P. Appanah, R. Lee, S. Tachibana, M. Shinkai, Y. Lehnertz, B. Mager, D. Rossi, F. and Lorincz, M.C.*. Lysine methyltransferase G9a is required for de novo DNA methylation and establishment but not maintenance of proviral silencing. Proc Natl Acad Sci U S A., 2011, 108:5718-5723. [PubMed]
      3. Karimi, M.M. Goyal, P. Maksakova, I.A. Bilenky, M. Leung, D. Tang, J.X. Shinkai, Y. Mager, D.L. Jones, S. Hirst M. and Lorincz, M.C*. DNA methylation and SETDB1/H3K9me3 regulate predominantly distinct sets of genes, retroelements and chimaeric transcripts in mouse ES cells. Cell Stem Cell. 2011, 8:676-687. [PubMed]
      4. Hayashi-Takanaka, Y. Yamagata, K. Wakayama, T. Stasevich, T.J. Kainuma, T. Tsurimoto, T. Tachibana, M. Shinkai, Y. Kurumizaka, H. Nozaki, N. and Kimura, H*. Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. Nucleic Acids Res. 2011, 39:6475-6488. [PubMed]
      5. Takada, Y. Naruse, C. Costa, Y. Shirakawa, T. Tachibana, M. Sharif, J. Kezuka-Shiotani, F. Kakiuchi, D. Masumoto, H. Shinkai, Y. Ohbo, K. Peters, A.H.F.M. Turner, J.M.A. Asano, M. and Koseki, H*. HP1· links histone methylation marks to meiotic synapsis in mice. Development, 2011, 138:4207-4217. [PubMed]
      6. Chang, Y. Sun, L. Kokura, K. Horton, J.R. Fukuda, M. Espejo, A. Izumi, V. Koomen, J.M. Bedford, M.T. Zhang, X. Shinkai, Y. Fang, J. and Cheng, X*. MPP8 mediates the interactions between DNA methyltransferase Dnmt3a and H3K9 methyltransferase GLP/G9a. Nature Communications. Nature Commun., 2011, 2:533. [PubMed]

      2010

      1. Balemans, M*. Huibers, M. Eikelenboom, N. Kuipers, A. van Summeren, R. Pijpers, M. Tachibana, M. Shinkai, Y. van Bokhoven, H. and van der Zee, C.E.. Reduced exploration, increased anxiety, and altered social behavior: autistic-like features in Ehmt1+/- mice. Behav Brain Res., 2010, 208:47-55. [PubMed]
      2. Hashimoto, H. Takami, Y. Sonoda, E. Iwasaki, T. Iwano, H. Tachibana, M. Takeda, S. Nakayama, T. Kimura, H#. and Shinkai, Y#*. Histone H1 null vertebrate cells exhibit altered nucleosome architecture. Nucleic Acids Res., 2010, 38:3533-3545. [PubMed]
      3. Matsui, T#. Leung, D#. Miyashita, H. Maksakova, I.A. Miyachi, H. Kimura, H. Tachibana, M. Lorincz, M.C*. and Shinkai, Y*. Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET. Nature. 2010, 464:927-931. [PubMed]
      4. Chang, Y. Ganesh, T. Horton, J.R. Spannhoff, A. Liu, J. Sun, A. Zhang, X. Bedford, M.T. Shinkai, Y. Snyder, J.P. and Cheng, X*. Adding a lysine mimic in the design of potent inhibitors of histone lysine methyltransferases. Journal of Molecular Biology, 2010, 400:1-7. [PubMed]

      2009

      1. Shirato, H. Ogawa, S. Nakajima, K. Inagawa, M. Kojima, M. Tachibana, M. Shinkai, Y. and Takeuchi, T*. A Jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9. J. Biol. Chem., 2009, 284:733-739. [PubMed]
      2. Wen, B. Wu, H. Shinkai, Y. Irizarry, R.A. and Feinberg, A.P*. Large organized chromatin K9-modifications (LOCKs) distinguish differentiated from embryonic stem cells. Nat. Genet. 2009, 41:246-250. [PubMed]
      3. Link, P. Gangisetty, O.James, S. Woloszynska-Read, A. Tachibana, M. Shinkai, Y. Karpf, A*. Distinct Roles for Histone Methyltransferases G9a and GLP in Cancer Germ-Line Antigen Gene Regulation in Human Cancer Cells and Murine Embryonic Stem Cells. Molecular Cancer Research. 2009, 7:851-862. [PubMed]
      4. Inagaki, T#. Tachibana, M#. Magoori, K. Kudo, H. Tanaka, T. Okamura, M. Naito, M. Kodama, T. Shinkai, Y*. and Sakai, Y*. Obesity and metabolic syndrome in histone demethylase JHDM2a deficient mice. Genes to Cells, 2009, 14:991-1001. [PubMed]
      5. Okamoto K. and Shinkai, Y*. TRFH domain is critical for TRF1-mediated telomere stabilization. Cell Structure and Function, 2009, 34:71-76. [PubMed]
      6. Komai T. Iwanari, H. Mochizuki, Y. Hamakubo, T. and Shinkai, Y*. Expression of the mouse PR domain protein Prdm8 in the developing central nervous system. Gene Expression Patterns, 2009, 9:503-514. [PubMed]
      7. Yokochi T. Poduch K. Ryba T. Lu J. Hiratani T. Tachibana M. Shinkai Y.and Gilbert D.M*. G9a selectively represses a class of late-replicating genes at the nuclear periphery. Proc Natl Acad Sci U S A., 2009, 106:19363-19368. [PubMed]

      2008

      1. Rathert P. Dhayalan A. Murakami M. Zhang X. Tamas R. Jurkowska R. Komatsu Y. Shinkai Y. Cheng X. and Jeltsch A*. Identification of new non-histone targets of human G9a protein lysine methyltransferase using peptide arrays. Nat Chem Biol., 2008, 4:344-346. [PubMed]
      2. Kato, Y. Kato, M. Tachibana, M. Shinkai, Y. and Yamaguchi, M*. Characterization of Drosophila G9a in vivo and identification of genetic interactions. Genes to Cells, 2008, 13:703-722. [PubMed]
      3. Thomas, L.R. Miyashita, H. Cobb, R.M. Pierce S. Tachibana, M. Hobeika, E. Reth, M. Shinkai, Y. and Oltz, E.M*. Functional Analysis of Histone Methyltransferase G9a in B and T Lymphocytes. J.Immunol., 2008, 181:485-493. [PubMed]
      4. Okamoto K. Iwano T. Tachibana M. and Shinkai Y*. Distinct roles of TRF1 in the regulation of telomere structure and lengthening. J. Biol. Chem., 2008, 283:23981-23988. [PubMed]
      5. Tachibana M*. Matsumura Y. Fukuda M. Kimura H. and Shinkai Y*. G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. EMBO J. 2008, 27:2681-2690. [PubMed]
      6. Dong K.D. Maksakova I.A. Mohn F. Leung D. Appanah R. Lee S. Yang H.W. Mager D. Schbeler D. Tachiana M. Shinkai Y. and Lorincz M.C*. DNA methylation of retrotransposons in stem cells requires the histone-methyltransferase G9a but not its catalytic activity. EMBO J. 2008, 27:2691-2701. [PubMed]
      7. Epsztejn-Litman, S. Feldman, N. Abu-Remaileh, M. Shufaro, Y. Gerson, A. Ueda, J. Deplus, R. Fuks, F. Shinkai, Y. Cedar, H*. and Bergman, Y. De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes. Nat Struct Mol Biol., 2008, 15:1176-1183. [PubMed]

      2007

      1. Ikegami, K. Iwatani, M. Suzuki, M. Tachibana, M. Shinkai, Y. Tanaka, S. Greally, J.M. Yagi, S. Hattori, N. and Shiota, K*. Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells. Genes to Cells, 2007, 12:1-11. [PubMed]
      2. Tachibana, M*. Nozaki, M. Takeda, N. and Shinkai, Y*. Functional Dynamics of H3K9 Methylation During Meiotic Prophase Progression. EMBO J. 2007, 26:3346-3359. [PubMed]
      3. Seki, Y. Yamaji, M. Yabuta, Y. Sano, M. Shigeta, M. Tachibana, M. Matsui, Y. Saga, Y. Shinkai, Y. and Saitou, M*. A cellular dynamics associated with the genome-wide epigenetic reprogramming in primordial germ cells in mice. Development, 2007, 134:2627-2638. [PubMed]
      4. Hashimoto, H. Sonoda, E. Takami, Y. Kimura, H. Nakayama, T. Tachibana, M. Takeda, S. and Shinkai, Y*. Histone H1 variant, H1R is involved in DNA damage response. DNA repair, 2007, 6:1584-1595. [PubMed]
      5. Nishida, M. Kato, M. Kato, Y. Sasai, N. Ueda, J. Tachibana, M. Shinkai, Y. and Yamaguchi M*. Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a. Genes to Cells, 2007, 12:877-888. [PubMed]

      2006

      1. Oka, S. Masutani, H. Liu, W. Kwon, Y.W. Hirata, H. Shinkai, Y. Yamada, S. Nishinaka, Y. Nakamura, H. and Yodoi, J*. Impaired fatty acid utilization in Thioredoxin Binding Protein-2 (TBP-2) deficient mice: a unique animal model of Reye syndrome. The FASEB Journal, 2006, 20:121-123. [PubMed]
      2. Feldman, N. Gerson, A. Fang, J. Li, E. Zhang, Y. Shinkai, Y. Ceder, H. and Bergman, Y*. G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis. Nat. Cell Biol. 2006, 8:188-194. [PubMed]
      3. Sendai, Y*. Sawada, T. Urakawa, M. Shinkai, Y. Kubota, K. Hoshi, H. and Aoyagi, Y. [alpha]1,3-Galactosyltransferase-Gene Knockout in Cattle using a Single Targeting Vector with loxP Sequences and Cre-Expressing Adenovirus. Transplantation, 2006, 81:760-766. [PubMed]
      4. Ueda J. Tachibana, M. Ikura, T. and Shinkai, Y*. Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP. J. Biol. Chem. 2006 281:20120-20128. [PubMed]
      5. Chen, H. Yan, Y. Davidson, T.L. Shinkai, Y. and Costa, M*. Hypoxic stress induces dimethylated histone H3 lysine 9 through histone methyltranferase G9a in mammalian cells. Cancer Research. 2006, 66:9009-9016. [PubMed]
      6. Akiyama, K. Yusa, K. Hashimoto, H. Poonepalli, A. Hande, M.P. Kakazu, N. Takeda, J. Tachibana, M. and Shinkai, Y*. Rad54 is dispensable for the ALT pathway. Genes to Cells, 2006, 11:1305-1315. [PubMed]
      7. Shinkai, Y. Function of euchromatic histone H3K9 methyltransferases, G9a/GLP. Chromatin and Disease, Series: Subcellular Biochemistry, Vol. 41 Kundu, Tapas K.; Dasgupta, Dipak (Eds.) Springer, 2006. (review)

      2005

      1. Collins, R.E. Tachibana, M. Tamaru, H. Jia, D. Zhang, X. Selker, E.U. Shinkai, Y. and Cheng, X*. In vitro and in vivo analyses of a Phe/Tyr switch controlling product specificity of histone lysine methyltransferases. J Biol Chem. 2005, 280:5563-5570. [PubMed]
      2. Tachibana, M. Ueda, J. Fukuda, M, Takeda, N. Ohta, T. Iwanari, H. Sakihama, T. Kodama, T. Hamakubo, T. & Shinkai, Y*. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Gene Dev. 2005, 19:815-826. [PubMed]
      3. Kurahashi, T. Nomura, T. Kanei-Ishii, C. Shinkai, Y. and Ishii, S*. The Wnt-NLK signaling pathway inhibits A-Myb activity by inhibiting the association with coactivator CBP and methylating Histone H3. Mol Biol Cell. 2005, 16:4705-4713. [PubMed]
      4. Kanatsu-Shinohara, M. Ogonuki, N., Iwano, T., Lee, J., Kazuki, Y., Inoue, K., Miki, H., Takehashi, M., Toyokuni, S., Shinkai, Y., Oshimura, M., Ishino, F., Ogura, A. and Shinohara, T*. Genetic and epigenetic properties of mouse male germline stem cells during long-term culture. Development, 2005, 132:4155-4163. [PubMed]

      2004

      1. Iwano, T. Tachibana, M. Reth, M. and Shinkai, Y*. Importance of TRF1 for fun ctional telomere structure. J. Biol. Chem. 2004, 279:1442-1448. [PubMed]
      2. Osipovitch, O. Meade, A. Milley, R. Oestreich, K. Tachibana, M. Shinkai, Y. Krangel, M.S. and Oltz, E.M*. Targeted inhibition of V(D)J recombination by a histone methyltransferase. Nat. Immunol. 2004, 5:309-316. [PubMed]
      3. Ohhata, T. Tachibana, M. Tada, M. Tada, T. Sasaki, H. Shinkai, Y. and Sado, T*. X-inactivation is stably maintained in mouse embryos deficient for histone methyltransferase G9a. Genesis, 2004, 40:151-156. [PubMed]
      4. Katagiri, K. Ohnishi, N. Kabashima, K. Iyoda, T. Takeda, N. Shinkai, Y. Inaba, K. and Kinashi, T*. Crucial functions of the Rap1 effector molecule RAPL in lymphocyte and dendritic cell trafficking. Nat. Immunol. 2004, 5:1045 - 1051. [PubMed]

      2003

      1. Xin, Z. Tachibana, M. Guggiari, M. Heard, E. Shinkai, Y. and Wagstaff, J*. Role of histone methyltransferase G9a in CpG methylation of the Prader-Willi syndrome imprinting center. J. Biol. Chem. 2003, 278:14996-15000. [PubMed]
      2. Shimizu, T. Shinkai, Y. Ogi, T. Ohmori, H. and Azuma, T*. The absence of DNA polymerase · does not affect somatic hypermutation of the mouse immunoglobulin heavy chain gene. Immunology letters 2003, 86:265-270. [PubMed]
      3. Fujita, N. Watanabe, S. Ichimura, T. Tsuruzoe, S. Shinkai, Y. Tachibana, M. Chiba, T.and Nakao, M*. MBD1 interacts with Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression. J. Biol. Chem. 2003, 278:24132-24138. [PubMed]
      4. Senoo, M. Wang, L. Suzuki, D. Takeda, N. Shinkai, Y. and Habu, S*. Increase of TCR Vbeta accessibility within Ebeta regulatory region influences its recombination frequency but not allelic exclusion. J. Immunol. 2003, 171:829-835. [PubMed]
      5. Cao, S. Bendall, H. Hicks, G.G. Nashabi, A. Sakano, H. Shinkai, Y. Gariglio, M. Oltz, E.M. and Ruley, H.E*. The HMG box protein Ssrp1/T160 is essential for cell viability in E3.5 mouse embryos. Mol. Cell. Biol. 2003, 23:5301-5307. [PubMed]
      6. Inouye, S. Katsuki, K. Izu, H. Fujimoto, M. Sugahara, K, Yamada, S. Shinkai, Y. Oka, Y. Katoh, Y. and Nakai, A*. Activation of heat shock genes is not necessary for heat shock transcription factor 1 to protect cell death against a single exposure to high temperatures. Mol. Cell. Biol. 2003, 23:5882-5895. [PubMed]
      7. Araki, Y*. Nohara, M. Yoshida-Komiya, H. Ogasawara, R. Kuramochi, T. Ito, M. Hoshi, H. Shinkai, Y. and Sendai, Y. Effect of a null mutation of the oviduct-specific glycoprotein gene on mouse reproduction. Biochem. J. 2003, 374:551-557. [PubMed]
      8. Sendai, Y. Sawada, T*. Urakawa, M. Shinkai, Y. Kubota, K. Teraoka, S. and Aoyagi, Y. Heterozygous disruption of the alpha1,3-galactosyltransferase gene in cattle. Transplantation 2003, 76:900-902. [PubMed]
      9. Rice, J.C. Briggs, S.D. Ueberheide, B. Barber, C.M. Shabanowitz, J. Hunt, D.F. Shinkai, Y. and Allis, C.D*. Mammalian histone methyltransferases direct different degrees of histone H3 lysine 9 methylation to define distinct domains of silent chromatin. Mol. Cell 2003, 12:1591-1598. [PubMed]
      10. Peters, A. Kubicek, S. Mechtler, K. O' Sullivan, R.J. Derijck, A.A. Perez-Burgos, L. Kohlmaier, A. Opravil, S. Tachibana, M. Shinkai, Y. Martens, J.H. and Jenuwein, T*. Partitioning and plasticity of repressive histone methylation states in mammalian chromatin. Mol. Cell 2003, 12:1577-1589. [PubMed]

      2002

      1. Shimozawa, N. Ono, Y. Muguruma, K. Hioki, K. Araki, Y. Shinkai, Y. Kono, T. and Ito, M. Direct production of gene-targeted mice from ES cells by nuclear transfer and the transmission to their progeny. Exp. Anim.2002, 51:375-381. [PubMed]
      2. Shinkai, Y*. Satoh, H. Takeda, N. Fukuda, M. Chiba, M. Kato, T. Kuramochi, T. and Araki, Y. A testicular germ cell-associated serine-threonine kinase, MAK is dispensable for sperm formation. Mol. Cell. Biol. 2002, 22:3276-3280. [PubMed]
      3. Tachibana, M. Sugimoto, K. Nozaki, M. Ueda, J. Ohta, T. Ohki, M. Fukuda, M. Takeda, N. Niida, H. Kato, H. and Shinkai, Y*. G9a histone methyltransferase plays a dominant role for euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes and Development, 2002 16:1779-1791. [PubMed]
      4. Shimozawa, N. Ono, Y. Kimoto, K. Muguruma, K. Sotomaru, Y. Hioki, K. Araki, Y. Shinkai, Y. Kono, T. and Ito, M. Abnormalities in cloned mice are not transmitted to the progeny. Genesis 2002 34:203-207. [PubMed]
      5. Ogi, T. Shinkai, Y. Tanaka, K. and Ohmori, H*. Pol· protects mammalian cells against the lethal and mutagenic effects of benzo·apyrene. Proc. Nath. Acad. Sci. U.S.A. 2002, 99:15548-15553. [PubMed]

      2001

      1. Kurita, A. Takizawa, T. Takayama, T, Totsukawa, K. Matsubara, S. Shibahara, H. Orgebin-Crist, M.-C. Sendo, F. Shinkai, Y. and Araki, Y*. Identification, cloning and initial characterization of a novel mouse testicular germ cell-specific antigen. Biol. Reprod. 2001, 64:935-945. [PubMed]
      2. Tachibana, M. Sugimoto, K. Fukushima, T. and Shinkai, Y*. SET-domain containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3. J. Biol. Chem. 2001, 276:25309-25317. [PubMed]
      3. Senoo, M. Mochida, N. Matsuura, Y. Suzuki, D. Takeda, N. Shinkai, Y*. and Habu, S*. Distinct regulation of V versus DJ transcription by the TCR enhancer: implication for Allelic Exclusion. Int. Immunol. 2001, 13:1405-1414. [PubMed]
      4. Tachibana, M. Narumi, O. Muguruma, K. Yamamoto, I. Shinkai, Y. and Yokota, Y*. Genomic organization and chromosomal mapping of the basic helix-loop-helix factor OUT (Tcf23/TCF23). Cytogenet. Cell. Genet. 2001, 94:23-25. [PubMed]
      5. Ono, Y. Shimozawa, N. Kimoto, S. Tachibana, M. Shinkai, Y. Ito, M. and Kono, T*. Production of cloned mice from embryonic stem cells arrested at metaphase. Reproduction 2001, 122:731-736. [PubMed]
      6. Shirane, M. Sawa, H. Kobayashi, Y. Nakano, T. Kitajima, K. Shinkai, Y. Nagashima, K. and Negishi, I*. Deficiency of phospholipase C-1 impairs renal development and hematopoiesis. Development 2001, 128:5173-5180. [PubMed]

      Publications -2000

      2000

      1. Niida, H. Shinkai, Y*. Hande, M.P. Matsumoto, T. Takehara, S. Tachibana, M. Oshimura, M. Lansdorp, P.M. and Furuichi, Y. Telomere maintenance in telomerase-deficient mouse embryonic stem cells: characterization of an amplified telomeric DNA. Mol. Cell. Biol. 2000, 20:4115-4127. [PubMed]
      2. Muramatsu, M. Kinoshita, K. Fagarasan, S. Yamada, S. Shinkai, Y. and Honjo, T*. Class switch recombination and hypermutation require Activation-Induced cytidine Deaminase (AID), a potential RNA editing enzyme. Cell ,2000, 102:553-563. [PubMed]
      3. Wakayama, T. Shinkai, Y. Tamashiro, K.L.K. Niida, H. Blanchard, C.D. Blanchard, R.J. Ogura, A. Tanemura, K. Tachibana, M. Perry, A.C.F. Monbaerts, P. and Yanagimachi, R*. Cloning of mice to six generations. Nature 2000, 407:318-319. [PubMed]
      4. Ramakrishnan, S., Eppenberger, U., Mueller, H., Shinkai, Y. and Narayanan, R*. Expression profile of the putative catalytic subunit of the telomerase gene. Cancer Res. 1998 58:622-625. [PubMed]
      5. Senoo, M. and Shinkai, Y*. Regulation of V germ-line transcription in RAG-deficient mice by the CD3-mediated signals; implication of V transcriptional regulation in TCR allelic exclusion. Int. Immunol. 1998 10:553-560. [PubMed]
      6. Niida, H. Matsumoto, T. Satoh, H. Shiwa, M, Tokutake, Y. Furuichi, Y. and Shinkai, Y*. Severe growth defect in mouse cells lacking a telomerase RNA component. Nature Genetics. 1998 19:203-206. [PubMed]
      7. Senoo, M. Seki, N. Ohira, M. Sugano, S. Watanabe, M. Tachibana, M. Tanaka, T. Shinkai, Y*. and Kato, H*. A second p53-related protein, p73L, with high homology to p73. A second p53-related protein, p73L, with high homology to p73. Biochem. Biophys. Res. Commun. 1998 248:603-607. [PubMed]
      8. Suzuki, H., Shinkai, Y., Granger, L.G., Alt, F.W., Love, P.E., and Singer, A*. Commitment of immature CD4+8+ thymocytes to the CD4 lineage requires CD3 signaling but does not require expression of clonotypic T cell receptor (TCR) chains. J. Exp. Med. 1997 186:17-23. [PubMed]
      9. Swat, W., Shinkai, Y., Cheng, H.L., Davidson, L. and Alt, F.W*. Activated Ras signals differentiation and expansion of CD4+8+ thymocytes. Proc. Natl. Acad. Sci. USA. 1996 93:4683-4687. [PubMed]
      10. Shinkai, Y.*, Ma, A., Cheng, H.L. and Alt, F.W. CD3 epsilon and CD3 zeta cytoplasmic domains can independently generate signals for T cell development and function. Immunity 1995, 2:401-411. [PubMed]
      11. Sen, J*., Venkataraman, L., Shinkai, Y., Pierce, J.W., Alt, F.W. Burakoff, S.J. and Sen, R. Expression and induction of nuclear factor-kappa B-related proteins in thymocytes. J. Immunol. 1995, 154:3213-3221. [PubMed]
      12. Karasuyama, H., Rolink, A., Shinkai, Y., Young, F., Alt, F. W., Melchers, F. The expression of VpreB/5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice. Cell 1994, 77:133-143. [PubMed]
      13. Young, F., Ardman, B., Shinkai, Y., Lansford, R., Blackwell, K.T., Mendelsohn, M., Rolink, A., Melchers, F. and Alt, F.W*. Influence of Immunoglobulin heavy and light chain expression on B cell differentiation. Genes and Development 1994, 8:1043-1057. [PubMed]
      14. Kojima, H., Shinohara, N*., Hanaoka, S., Someya-Shirota, Y., Takagaki, Y., Ohno, H., Saito, T., Katayama, T., Yagita, H., Okumura, K., Shinkai, Y., Alt, F.W., Matsuzawa, A., Yonehara, S. and Takayama, H. Two distinct pathways of specific killing revealed by perforin mutant cytotoxic T lymphocytes. Immunity 1994, 1:357-364. [PubMed]
      15. Shinkai, Y. and Alt. F. W*. CD3 chain-mediated signals rescue the development of CD4-8- to CD4+8+ thymocytes in RAG2-/- mice in the absence of TCR beta chain expression. Int. Immunol. 1994 6:995-1001. [PubMed]
      16. Sen, J*., Shinkai, Y., Alt, F.W., Sen R. and Burakoff, S.J. Nuclear factors that mediate intrathymic signals are developmentally regulated. J. Exp. Med. 1994 180:2321-2327. [PubMed]
      17. Seko, Y., Minota, S., Kawasaki, A., Shinkai, Y., Maeda, K., Yagita, H., Okumura, K., Sato, O., Takagi, A., Tada, Y. and Yazaki, Y. Perforin-secreting killer cell infiltration and expression of a 65-kD heat-shock protein in aortic tissue of patients with Takayasu arthritis. J. Clin. Invest. 1994 93:750-758. [PubMed]
      18. Shinkai, Y., Koyasu, S., Nakayama, K. Murphy, K. Loh, D. Y., Reinherz, E.L. and Alt. F. W*. Restoration of T cell development in RAG-2 deficient mice by functionally rearranged TCR transgenes. Science 1993; 259:822-825. [PubMed]
      19. Rodewald, H.-R., Awad, K. Moigeon, P. D'Adamio, L. Rabinowitz, D. Shinkai, Y., Alt, F.W. and Reinherz. E.L*. Fc_RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor-b-chain rearrangement status. J. Exp. Med. 1993; 177:1079-1092. [PubMed]
      20. Nakayama, K., Nakayama, K., Negishi, I., Kuida, K., Shinkai, Y., Marjorie C. L., Fields, L.E., Lucas, J. P., Stewart, V., Alt, F. W. and Loh, D. Y*. Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice. Science 1993; 261:1584-1588. [PubMed]
      21. Seko, Y., Yamazaki, T., Shinkai, Y., Yagita, H., Okumura, K., Naito, S., Imataka, K., Fujii, J. and Yazaki, Y*. Cellular and molecular bases for the immunopathology of the myocardial cell damage involved in acute viral myocarditis with special reference to dilated cardiomyopathy. Jpn. Cir. J. 1992; 56:1062-1072. [PubMed]
      22. Kawasaki, A., Shinkai, Y., Yagita, H. and Okumura, K*. Expression of perforin in murine natural killer cells and cytotoxic T lymphocytes in vivo. Eur. J. Immunol. 1992; 22:1215-1219. [PubMed]
      23. Shinkai, Y., Rathbun, G., Lam, K-P., Oltz, E.M., Stewart, V., Mendelsohn, M., Charron, J., Datta, M., Young, F., Stall, A.M. and Alt, F.W*. RAG-2 deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell 1992; 68:855-867. [PubMed]
      24. Takeuchi, Y., Nishimura, T., Gao, XH., Watanabe, K., Akatsuka, A., Shinkai, Y., Okumura, K. and Habu, S. Perforin is expressed in CTL populations generated in vivo. Immunology letters 1992; 31:183-187. [PubMed]
      25. Taccioli, G.E., Rathbun, G. Shinkai, Y., Oltz, E.M., Cheng, H., Whitmore, G., Stamato, T., Jeggo, P. and Alt, F.W*. Activities involved in V(D)J recombination. Current Topics in Microbiology and Immunology 1992; 182:107-114. (review) [PubMed]
      26. Nakata, M., Kawasaki, A., Azuma, M., Tsuji, Matsuda, H., Shinkai, Y., Yagita, H. and Okumura, K*. Expression of perforin and cytolytic potential of human peripheral blood lymphocyte subpopulations. Int. Immunol. 1992; 4:1049-1054. [PubMed]
      27. Takayama, H., Shinohara, N*., Kawasaki, A., Someya, Y., Hanaoka, S., Kojima, H., Yagita, H., Okumura, K. and Shinkai, Y. Antigen-specific directional target cell lysis by perforin-negative T lymphocyte clones. Int. Immunol. 1991; 3:1149-1156 [PubMed]
      28. Seko, Y., Shinkai, Y., Kawasaki, A., Yagita, H., Okumura, K. Takaku, F. and Yazaki, Y*. Expression of perforin in infiltrating cells in murine hearts with acute myocarditis caused by coxsakievirus B3.Circulation 1991; 84:788-795. [PubMed]
      29. Oshimi, K*., Shinkai, Y., Okumura, K., Oshimi, Y and Mizoguchi, H. Perforin gene expression in granular lymphocyte proliferative disorders. Blood 1990; 75:704-708. [PubMed]
      30. Smyth, M. J., Ortaldo, J. R., Shinkai, Y., Yagita, H., Nakata, M., Okumura, K and Young, H. A*. Interleukin 2 induction of pore-forming protein gene expression in human peripheral blood CD8+ T cells. J. Exp. Med. 1990; 171:1269-1281. [PubMed]
      31. Kawasaki, A., Shinkai, Y., Kuwana, Y., Furuya, A., Iigo, Y., Hanai, N., Itoh, S., Yagita, H. and Okumura, K*. Perforin, a pore-forming protein detectable by monoclonal antibodies, is a functional marker for killer cells. Int. Immunol. 1990; 2:677-684. [PubMed]
      32. Kobata, T., Shinkai, Y., Iigo, Y., Kawasaki, A., Yagita, H., Itoh, S., Shimada, S., Katz, S. I. and Okumura, K*. Thy-1-positive dendritic epidermal cells contain a killer protein perforin. Int. Immunol. 1990; 2:1113-1116 [PubMed]
      33. Nakata, M., Smyth, M. J., Norihisa, Y., Kawasaki, A., Shinkai, Y., Okumura, K*. and Yagita, H. Constitutive expression of pore-forming protein in peripheral blood_ T cells: Implication for their cytotoxic role in vivo. J. Exp. Med. 1990; 172:1877-1880. [PubMed]
      34. Nakayama, K., Shinkai, Y., Okumura, K and Nakauchi, H*. Isolation and characterization of the mouse CD8 b-chain (Ly-3) genes: Absence of an intervening sequence between V- and J-like gene segments. J. Immunol. 1989; 142:2540-2546. [PubMed]
      35. Ishikawa, H., Shinkai, Y., Yagita, H., Yue, C. C., Henkart, P. A., Sawada, S., Young, H. A., Reynolds, C. W. and Okumura, K*. Molecular cloning of rat cytolysin. J. Immunol. 1989; 143:3069-3073. [PubMed]
      36. Shinkai, Y., Yoshida, C. M., Maeda, K., Kobata, T., Maruyama, K. Yodoi, J., Yagita, H and Okumura K*. Molecular cloning and chromosomal assignment of human perforin gene. Immunogenetics 1989; 30:452-457. [PubMed]
      37. Shinkai, Y., Ishikawa, H., Hattori, M. and Okumura, K*. Resistance of mouse cytolytic cells to pore-forming protein (PFP)-mediated cytolysis. Eur. J. Immunol. 1988; 18:29-33. [PubMed]
      38. Shinkai, Y., Nakauchi, H., Honjo, T. and Okumura, K*. Mouse immunoglobulin allotypes: Multiple differences between the nucleic acid sequences of the IgEa and IgEb alleles. Immunogenetics 1988; 27:288-292. [PubMed]
      39. Shinkai, Y. Takio, K. and Okumura, K*. Homology of perforin to the ninth component of complement (C9). Nature 1988; 334:525-527. [PubMed]
      40. Nakauchi, H*., Shinkai, Y. and Okumura, K. Molecular cloning of Lyt-3, a membrane glycoprotein marking a subset to mouse cell lymphocytes: Molecular homology to immunoglobulin and T-cell receptor variable and joining regions. Proc. Nath. Acad. Sci. U.S.A. 1987; 84:4210-4214. [PubMed]

*corresponding author

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