Faculty of Agriculture, The University of Tokyo, Japan, B. S., 2002
2002-2007
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. Ph. D., 2007
Research and Professional Experience
2007-2011
Postdoctoral Fellow, University of California, San Francisco, U.S.A.
2011
Postdoctoral Researcher, RIKEN, Japan
2011- 2016
Staff Scientist, RIKEN, Japan
2016- present
Senior Research Scientist, RIKEN, Japan
Awards and Professional Activities
2020
JSPS GIA for Scientific Research (C)
2016
Inamori Grant Program, JSPS GIA for YS-B
2015
Takeda Science Foundation, The Naito Foundation, The Uehara Memorial Foundation, The Mochida Memorial Foundation for Medical and Pharmaceutical Research
2012
Fund for Seeds of Collaborative Research, RIKEN
2004-
Member, The Molecular Biology Society of Japan
2015-
Member, The Japanese Society for Epigenetics
Publication List
Shimazu T#*. Yoshimoto R#. Kotoshiba K. Suzuki T. Matoba S. Hirose M. Akakabe M. Sohtome Y. Sodeoka M. Ogura A. Dohmae N. Shinkai Y*. Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism. Genes & Development. 2023 Aug 23. doi: 10.1101/gad.350755.123. [PubMed]
Matsuura-Suzuki E#. Shimazu T#*. Takahashi M. Kotoshiba K. Suzuki T. Kashiwagi K. Dohmae N. Ito T*. Shinkai Y*. Iwasaki S*. METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance. eLife 2022 Jun 8;11:e72780. doi: 10.7554/eLife.72780. [PubMed]
Sohtome Y*. Shimazu T*. Shinkai Y*. Sodeoka M*. Propargylic Se-adenosyl-l-selenomethionine: A Chemical Tool for Methylome Analysis. Acc Chem Res.2021 Oct 19;54(20):3818-3827. [PubMed]
Davydova E#. Shimazu T#. Schuhmacher M.K. Jakobsson M.E. Willemen H.L.D.M. Liu T. Moen A. Ho A.Y.Y. Małecki J. Schroer L. Pinto R. Suzuki T. Gronsberg I.A. Sohtome Y. Akabane M. Weirich S. Kikuchi M. Olsen J.V. Dohmae N. Umehara T. Sodeoka M. Siino V. McDonough M. Eijkelkamp N. Schofield C.J. Jeltsch A*. Shinkai Y*. Falnes P.O*. The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. Nat Commun. 2021, 12:891. [PubMed]
Tsusaka T, Shimura C, Shinkai Y*. ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination. EMBO reports. [PubMed]
Sohtome, Y., Shimazu, T., Barjau, J., Fujishiro, S., Akakabe, M., Terayama, N., Dodo, K., Ito, A., Yoshida, M., Shinkai, Y., and Sodeoka, M. (2018). Unveiling epidithiodiketopiperazine as a non-histone arginine methyltransferase inhibitor by chemical protein methylome analyses. Chem Commun (Camb), 54(66): 9202-9205. PMID: 30063235 [PubMed]
Shimazu T, Furuse T, Balan S, Yamada I, Okuno S, Iwanari H, Suzuki T, Hamakubo T, Dohmae N, Yoshikawa T, Wakana S, Shinkai Y*. Role of METTL20 in regulating β-oxidation and heat production in mice under fasting or ketogenic conditions.Sci Rep. 2018 Jan 19;8(1):1179 [PubMed]
Ferry L#, Fournier A#, Tsusaka T#, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y*, Defossez PA*. Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation. Mol Cell. 2017 Aug 17;67(4):550-565.e5. #These authors contributed equally [PubMed]
Shirakawa, K., Wang, L., Man, N., Maksimoska, J., Sorum, A. W., Lim, H. W., Lee, I. S., Shimazu, T., Newman, J. C., Schroder, S., Ott, M., Marmorstein, R., Meier, J., Nimer, S., and Verdin, E. (2016). Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife, 5: e11156. PMID: 27244239 26602019
Shimazu, T. Barjau, J. Sohtome, Y. Sodeoka, M. and Shinkai, Y. Selenium-based S-adenosylmethionine analog reveals the mammalian seven-beta-strand methyltransferase METTL10 to be an EF1A1 lysine methyltransferase. PLOS ONE 2014 Aug 21;9(8):e105394. doi: 10.1371/journal.pone.0105394.[PubMed]
Shimazu, T., M.D. Hirschey, J. Newman, W. He, K. Shirakawa, N. Le Moan, C.A. Grueter, H. Lim, L.R. Saunders, R.D. Stevens, C.B. Newgard, R.V. Farese, Jr., R. de Cabo, S. Ulrich, K. Akassoglou, and E. Verdin (2013). Suppression of oxidative stress by beta-hydroxybutyrate, an endogenous histone deacetylase inhibitor. Science. 339:211-4. PMID: 23223453
Takahashi, M., Y. Takemoto, T. Shimazu, H. Kawasaki, M. Tachibana, Y. Shinkai, M. Takagi, K. Shin-ya, Y. Igarashi, A. Ito, and M. Yoshida (2012). Inhibition of histone H3K9 methyltransferases by gliotoxin and related epipolythiodioxopiperazines. J Antibiot (Tokyo). 65:263-5. PMID: 22334240
Cho, H.S., T. Shimazu, G. Toyokawa, Y. Daigo, Y. Maehara, S. Hayami, A. Ito, K. Masuda, N. Ikawa, H.I. Field, E. Tsuchiya, S. Ohnuma, B.A. Ponder, M. Yoshida, Y. Nakamura, and R. Hamamoto (2012). Enhanced HSP70 lysine methylation promotes proliferation of cancer cells through activation of Aurora kinase B. Nat Commun. 3:1072. PMID: 22990868
Hirschey, M.D., T. Shimazu, E. Jing, C.A. Grueter, A.M. Collins, B. Aouizerat, A. Stancakova, E. Goetzman, M.M. Lam, B. Schwer, R.D. Stevens, M.J. Muehlbauer, S. Kakar, N.M. Bass, J. Kuusisto, M. Laakso, F.W. Alt, C.B. Newgard, R.V. Farese, Jr., C.R. Kahn, and E. Verdin (2011). SIRT3 deficiency and mitochondrial protein hyperacetylation accelerate the development of the metabolic syndrome. Mol Cell. 44:177-90. PMID: 21856199
Hirschey, M.D., T. Shimazu, J.A. Capra, K.S. Pollard, and E. Verdin (2011). SIRT1 and SIRT3 deacetylate homologous substrates: AceCS1,2 and HMGCS1,2. Aging (Albany NY). 3:635-42. PMID: 21701047
Shimazu, T., M.D. Hirschey, J.Y. Huang, L.T. Ho, and E. Verdin (2010). Acetate metabolism and aging: An emerging connection. Mech Ageing Dev. 131:511-6. PMID: 20478325
Shimazu, T., M.D. Hirschey, L. Hua, K.E. Dittenhafer-Reed, B. Schwer, D.B. Lombard, Y. Li, J. Bunkenborg, F.W. Alt, J.M. Denu, M.P. Jacobson, and E. Verdin (2010). SIRT3 deacetylates mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase 2 and regulates ketone body production. Cell Metab. 12:654-61. PMID: 21109197
Huang, J.Y., M.D. Hirschey, T. Shimazu, L. Ho, and E. Verdin (2010). Mitochondrial sirtuins. Biochim Biophys Acta. 1804:1645-51. PMID: 20060508
Hirschey, M.D., T. Shimazu, E. Goetzman, E. Jing, B. Schwer, D.B. Lombard, C.A. Grueter, C. Harris, S. Biddinger, O.R. Ilkayeva, R.D. Stevens, Y. Li, A.K. Saha, N.B. Ruderman, J.R. Bain, C.B. Newgard, R.V. Farese, Jr., F.W. Alt, C.R. Kahn, and E. Verdin (2010). SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature. 464:121-5. PMID: 20203611
Hirschey, M.D., T. Shimazu, J.Y. Huang, and E. Verdin (2009). Acetylation of mitochondrial proteins. Methods Enzymol. 457:137-47. PMID: 19426866
Kamemura, K., A. Ito, T. Shimazu, A. Matsuyama, S. Maeda, T.P. Yao, S. Horinouchi, S. Khochbin, and M. Yoshida (2008). Effects of downregulated HDAC6 expression on the proliferation of lung cancer cells. Biochem Biophys Res Commun. 374:84-9. PMID: 18602369
Shimazu, T., S. Horinouchi, and M. Yoshida (2007). Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3'-end processing. J Biol Chem. 282:4470-8. PMID: 17172643
Tomono, A., M. Mashiko, T. Shimazu, H. Inoue, H. Nagasawa, M. Yoshida, Y. Ohnishi, and S. Horinouchi (2006). Self-activation of serine/threonine kinase AfsK on autophosphorylation at threonine-168. J Antibiot (Tokyo). 59:117-23. PMID: 16629414
Shimazu, T., Y. Komatsu, K.I. Nakayama, H. Fukazawa, S. Horinouchi, and M. Yoshida (2006). Regulation of SV40 large T-antigen stability by reversible acetylation. Oncogene. 25:7391-400. PMID: 16767160
Masumi, A., H. Fukazawa, T. Shimazu, M. Yoshida, K. Ozato, K. Komuro, and K. Yamaguchi (2006). Nucleolin is involved in interferon regulatory factor-2-dependent transcriptional activation. Oncogene. 25:5113-24. PMID: 16582966
Yoshida, M., T. Shimazu, and A. Matsuyama (2003). Protein deacetylases: enzymes with functional diversity as novel therapeutic targets. Prog Cell Cycle Res. 5:269-78. PMID: 14593721
Matsuyama, A., T. Shimazu, Y. Sumida, A. Saito, Y. Yoshimatsu, D. Seigneurin-Berny, H. Osada, Y. Komatsu, N. Nishino, S. Khochbin, S. Horinouchi, and M. Yoshida (2002). In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. EMBO J. 21:6820-31. PMID: 12486003
