Akeo Shinkai

Akeo Shinkai, Ph.D

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    School of Agriculture, Nagoya University, 1986
    Graduate School of Agriculture, Nagoya University, 1988
    Graduate School of Agriculture, The University of Tokyo, 1991 (Doctor of Agriculture)

    Research and Professional Experience

    Research Scientist, Tokyo Research Laboratories, Kyowa Hakko Kogyo, Co., Ltd.
    Visiting Scholar, Department of Pathology and Biochemistry, University of Washington
    Part-time Lecturer, Waseda University
    Senior Research Scientist, RIKEN
    Team Leader, RIKEN
    Part-time Lecturer, Osaka University

    Awards and Professional Activities

    Member, The Molecular Biology Society of Japan
    Member, The Japanese Biochemical Society
    Member, The Japanese Society for Epigenetics

    Selected publication List

    1. Taylor DW#, Zhu Y#, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E*, and Doudna JA*.
      Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.
      Science. 2015 May 1;348(6234):581-585. [PubMed]
    2. Staals RH*, Zhu Y, Taylor DW, Kornfeld JE, Sharma K, Barendregt A, Koehorst JJ, Vlot M, Neupane N, Varossieau K, Sakamoto K, Suzuki T, Dohmae N, Yokoyama S, Schaap PJ, Urlaub H, Heck AJ, Nogales E, Doudna JA, Shinkai A, and van der Oost J*.
      RNA targeting by the Type III-A CRISPR-Cas Csm complex of Thermus thermophilus.
      Mol Cell. 2014 Nov 20;56(4):518-530. [PubMed]
    3. Staals RH#, Agari Y#, Maki-Yonekura S#, Zhu Y, Taylor DW, van Duijn E, Barendregt A, Vlot M, Koehorst JJ, Sakamoto K, Masuda A, Dohmae N, Schaap PJ, Doudna JA, Heck AJ, Yonekura K, van der Oost J*, and Shinkai A*.
      Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus.
      Mol Cell. 2013 Oct 10;52(1):135-145. [PubMed]
    4. Agari Y, Sakamoto K, Yutani K, Kuramitsu S, and Shinkai A*.
      Structure and function of a TetR family transcriptional regulator, SbtR, from Thermus thermophilus HB8.
      Proteins. 2013 Jul;81(7):1166-1178. [PubMed]
    5. Agari Y, Sakamoto K, Kuramitsu S, and Shinkai A*.
      Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8.
      J Bacteriol. 2012 Sep;194(17):4630-4641. [PubMed]
    6. Sakamoto K, Agari Y, Kuramitsu S, and Shinkai A*.
      Phenylacetyl coenzyme A is an effector molecule of the TetR family transcriptional repressor PaaR from Thermus thermophilus HB8.
      J Bacteriol. 2011 Sep;193(17):4388-4395. [PubMed]
    7. Agari Y, Agari K, Sakamoto K, Kuramitsu S, and Shinkai A*.
      TetR-family transcriptional repressor Thermus thermophilus FadR controls fatty acid degradation.
      Microbiology. 2011 Jun;157(Pt 6):1589-1601. [PubMed]
    8. Sakamoto K#, Agari Y#, Agari K, Kuramitsu S, and Shinkai A*.
      Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8.
      Microbiology. 2010 Jul;156(Pt 7):1993-2005. [PubMed]
    9. Agari Y, Sakamoto K, Tamakoshi M, Oshima T, Kuramitsu S, and Shinkai A*.
      Transcription profile of Thermus thermophilus CRISPR systems after phage infection.
      J Mol Biol. 2010 Jan 15;395(2):270-281. [PubMed]
    10. Agari Y, Kashihara A, Yokoyama S, Kuramitsu S, and Shinkai A*.
      Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator.
      Mol Microbiol. 2008 Oct;70(1):60-75. [PubMed]
    11. Sakamoto K#, Agari Y#, Yokoyama S, Kuramitsu S, and Shinkai A*.
      Functional identification of an anti-sigmaE factor from Thermus thermophilus HB8.
      Gene. 2008 Nov 1;423(2):153-159. [PubMed]
    12. Shinkai A*, Ohbayashi N, Terada T, Shirouzu M, Kuramitsu S, and Yokoyama S*.
      Identification of promoters recognized by RNA polymerase-sigmaE holoenzyme from Thermus thermophilus HB8.
      J Bacteriol. 2007 Dec;189(23):8758-8764. [PubMed]
    13. Shinkai A, Sekine S, Urushibata A, Terada T, Shirouzu M, and Yokoyama S*.
      The putative DNA-binding protein Sto12a from the thermoacidophilic archaeon Sulfolobus tokodaii contains intrachain and interchain disulfide bonds.
      J Mol Biol. 2007 Oct 5;372(5):1293-1304. [PubMed]
    14. Shinkai A*, Kira S, Nakagawa N, Kashihara A, Kuramitsu S, and Yokoyama S.
      Transcription activation mediated by a cyclic AMP receptor protein from Thermus thermophilus HB8.
      J Bacteriol. 2007 May;189(10):3891-3901. [PubMed]
    15. Shinkai A*, Komuta-Kunitomo M, Sato-Nakamura N, and Anazawa H.
      N-terminal domain of eotaxin-3 is important for activation of CC chemokine receptor 3.
      Protein Eng. 2002 Nov;15(11):923-929.[PubMed]
    16. Shinkai A and Loeb LA*.
      In vivo mutagenesis by Escherichia coli DNA polymerase I.
      J Biol Chem. 2001 Dec 14;276(50):46759-46764. [PubMed]
    17. Shinkai A, Patel PH, and Loeb LA*.
      The conserved active site motif A of Escherichia coli DNA polymerase I is highly mutable.
      J Biol Chem. 2001 Jun 1;276(22):18836-18842. [PubMed]
    18. Shinkai A#, Yoshisue H#, Koike M, Shoji E, Nakagawa S, Saito A, Takeda T, Imabeppu S, Kato Y, Hanai N, Anazawa H, Kuga T, and Nishi T*.
      A novel human CC chemokine, eotaxin-3, which is expressed in IL-4-stimulated vascular endothelial cells, exhibits potent activity toward eosinophils.
      J Immunol. 1999 Aug 1;163(3):1602-1610. [PubMed]
    19. Shinkai A#, Ito M#, Anazawa H, Yamaguchi S, Shitara K*, and Shibuya M*.
      Mapping of the sites involved in ligand association and dissociation at the extracellular domain of the kinase insert domain-containing receptor for vascular endothelial growth factor.
      J Biol Chem. 1998 Nov 20;273(47):31283-31288. [PubMed]
    20. Shinkai A, Mei LH, Tokuda H, and Mizushima S*.
      The conformation of SecA, as revealed by its protease sensitivity, is altered upon interaction with ATP, presecretory proteins, everted membrane vesicles, and phospholipids.
      J Biol Chem. 1991 Mar 25;266(9):5827-5833. [PubMed]


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